Evidence of Co-Transcriptional Regulation at the X-linked Imprinted Locus, X-linked Lymphocyte Regulated 3b
Digital Document
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http://hdl.handle.net/11134/20002:860634147
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Persons
Author (aut): Qureshi, Sohaib M.
Thesis advisor (ths): O'Neill, Michael J
Associate Advisor (asa): Holsinger, Kent
Associate Advisor (asa): Pask, Andrew
Associate Advisor (asa): Mellone, Barbara
Associate Advisor (asa): O'Neill, Rachel J
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Title |
Title
Title
Evidence of Co-Transcriptional Regulation at the X-linked Imprinted Locus, X-linked Lymphocyte Regulated 3b
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Origin Information
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Parent Item
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Digital Origin
born digital
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Description |
Description
Genomic imprinting is defined as the mono-allelic expression of a gene based on parental origin. Eutherian mammals utilize a differentially methylated region, or DMR as an epigenetic mark to regulate transcription initiation events at all autosomal imprinted loci. However, an X-linked imprinted locus, X-linked lymphocyte regulated 3b, defies the classical mechanism of epigenetic regulation as no DMR has been identified. A recent finding at the Xlr3/4/5 locus shows that imprinted expression is only observed at the 3’ end of the Xlr3b primary transcript suggesting that paternal silencing is accomplished through a co-transcriptional mechanism. This study offers the first investigation into the potential imprinting mechanism by exploring the behavior of RNA Polymerase II (PolII) during transcription elongation. The observation that PolII is perturbed as it transits across the paternal copy of Xlr3b is further supported by H3K36me3 enrichment at the precise location of the stall, suggesting it too may play a role in the imprinting mechanism. The chromatin environment at the locus is not maintained by known allele specific histone modifications and histone methyltransferases that influence proper transcription elongation do not seem to play a role in the imprinting mechanism either. This work establishes a model where maternally biased gene expression is accomplished by severely impeding PolII at the paternal copy of Xlr3b during transcription elongation.
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Genre
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Organizations
Degree granting institution (dgg): University of Connecticut
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Rights Statement
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Use and Reproduction |
Use and Reproduction
These Materials are provided for educational and research purposes only. The University of Connecticut Library holds the copyright except where noted. Permission must be obtained in writing from the University of Connecticut Library and/or the owner(s) of the copyright to publish reproductions or quotations beyond "fair use."
These materials are provided for educational and research purposes only.
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Degree Name |
Degree Name
Doctor of Philosophy
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Degree Level |
Degree Level
Doctoral
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Degree Discipline |
Degree Discipline
Genetics and Genomics
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Local Identifier |
Local Identifier
ASC Theses 18388
841389035
OC_d_52
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