Characterization of Osteocyte Apoptosis in a Mouse Model of Orthodontic Tooth Movement
Digital Document
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http://hdl.handle.net/11134/20002:860636095
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Persons
Creator (cre): Moin, Sogole S.
Major Advisor (mja): Uribe, Flavio
Associate Advisor (asa): Nanda, Ravindra
Associate Advisor (asa): Kalajzic, Ivo
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Title |
Title
Title Non-Sort
The
Title
Characterization of Osteocyte Apoptosis in a Mouse Model of Orthodontic Tooth Movement
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Origin Information
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Parent Item
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Resource Type
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Digital Origin |
Digital Origin
born digital
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Description |
Description
Background: Osteocyte apoptosis is regularly observed in the alveolar bone during orthodontic tooth movement (OTM). In this study, we wanted to further investigate its occurrence and localization. Method: Sixteen 10-12 week old male CD1 mice were loaded with NiTi springs between the right first maxillary molar and incisor delivering 10-12g of force. Left hemimaxillae served as unloaded controls. Four mice were sacrificed at each of the following time points: 6 hours, 12 hours, 3 days and 7 days after loading. Apoptosis was detected by TUNEL assay and caspase-3 and RipK-1 were detected by immunostaining on histological sections. Results: The ratio of TUNEL-positive osteocytes was increased on the compression side at 12 hours and, to a lesser extent, 3 days. There was a trend for increased caspase-3 expression at 12 hours, but RipK-1 expression levels did not follow this same trend. Conclusions: Caspase-3 expression correlates with increased osteocyte apoptosis during OTM. These results provide a better understanding of the molecular mechanism of osteocyte death during OTM.
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Genre
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Organizations
Degree granting institution (dgg): University of Connecticut
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Rights Statement
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Use and Reproduction |
Use and Reproduction
These materials are provided for educational and research purposes only.
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Degree Name |
Degree Name
Master of Dental Science
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Degree Level |
Degree Level
Doctoral
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Degree Discipline |
Degree Discipline
Dental Science
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Local Identifier |
Local Identifier
OC_th_298
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